Discussion on the relationship between the active effects of Dendrobium and human health

The bioactive compounds of Dendrobium officinale have multiple biological properties such as antioxidant, anti-inflammatory and immunomodulatory, and have multiple health benefits such as anti-cancer, anti-diabetes, cardiovascular protection, gastrointestinal regulation, liver protection, lung protection and neuroprotection. Exploring the relationship between the active ingredients in Dendrobium officinale and human health is conducive to exploring the dose-effect relationship between Dendrobium officinale and human health, and improving the deep processing and industrialization of Dendrobium officinale.

Sharing of recent research results

Dendrobium officinale leaf polysaccharides have the dual effects of alleviating immunosuppressive mouse syndrome and regulating the immune system of normal mice

Abstract:

The purpose of this study is to explore the immunomodulatory effect of Dendrobium officinale leaf polysaccharides (DOLP) on immunosuppressive mice and normal mice. Experimental data showed that DOLP significantly reversed thymus and spleen damage in immunosuppressed mice, regulated the production of peripheral blood cells (PLT, etc.), immune-related cytokines (TNF-α, INF-γ and IL-6) and immunoglobulins (IgA, IgG and IgM), increased beneficial bacteria (Lactobacillus, Bifidobacterium, etc.), and reduced the pathogenicity of pathogenic bacteria (Desulfovibrio, etc.). In normal mice, DOLP significantly increased immune-related cytokines (TNF-α, INF-γ and IL-6), intestinal flora diversity and the relative abundance of beneficial flora (unclassified_f_Lachnospiraceae_NK4A136_group, etc.). The results showed that DOLP can exert immunomodulatory effects on both immunosuppressed mice and normal mice by regulating immune parameters and intestinal flora. These findings provide new insights into the immunomodulatory effects of DOLP and lay a solid foundation for the comprehensive utilization of DOLP.

Conclusions:

The effects of DOLP on immune regulation were detected by body weight, immune organ coefficient, peripheral blood cell count, immune-related cytokines and intestinal flora, and the effects of DOLP on the immune system of immunosuppressed mice and normal mice were explored. The results showed that DOLP could not only restore the body weight, abnormal immune organs and intestinal flora composition of immunosuppressed mice, reduce peripheral blood cell count and immune cytokine content, but also enhance immune stimulation response and significantly improve the immune cytokine level and intestinal flora diversity of normal mice. This indicates that DOLP has a good immunoregulatory effect, which can not only restore the immune function of immunosuppressed mice, but also regulate the immune function of normal mice. However, the exact mechanism by which DOLP exerts its immunoregulatory effect remains to be studied.

Protective effect of Dendrobium functional food on menopausal rats mediated by FSHR/PI3K/AKT signaling pathway

Abstract:

Menopausal symptoms are age-related diseases related to estrogen deficiency, which can be affected by emotions and eating habits. Guocaoyin (GSNF), a traditional Chinese medicine with Dendrobium officinale as the main ingredient, has significant efficacy in alleviating metabolic disorders caused by unhealthy diet. Currently, there are limited studies on the effects of GSNF on menopausal syndrome, and its potential pharmacological mechanisms are still unclear. This study aimed to evaluate the improvement of GSNF on menopausal syndrome and explore its effects on endocrine metabolic disorders caused by ovarian failure. The results showed that GSNF increased the levels of serum estradiol (E2), anti-Mullerian hormone (AMH), and inhibin B (INHB) in rats with menopausal syndrome, decreased the levels of serum gonadotropin-releasing hormone (GnRH) and follicle-stimulating hormone (FSH), and improved ovarian pathological damage. It was found that GSNF enhanced the expression of ovarian FSHR and ERα proteins, stimulated follicular development by activating the PI3K/AKT pathway, and inhibited follicular apoptosis by regulating the expression of apoptosis-related proteins Bcl-2, BAX, and Caspase-3, thereby improving ovarian function. In addition, GSNF enhanced the expression of hippocampal FSHR protein and upregulated the expression of hippocampal PI3K and AKT proteins. It reduces serum corticosterone (CORT) and adrenocorticotropic hormone (ACTH) levels, promotes the release of 5-hydroxytryptamine (5-HT), dopamine (DA) and norepinephrine (NE), and increases the number of neurons in hippocampal tissue, thereby improving depressive-like symptoms in menopause. Supplementation of GSNF can improve glucose and lipid metabolism disorders and alleviate liver steatosis in model rats. In summary, the study revealed a new function of GSNF in alleviating the adverse effects of negative emotions and unhealthy eating habits on menopausal symptoms. These findings suggest that GSNF may be a viable option for the treatment of menopausal syndrome.

Conclusions:

This experiment showed that GSNF had a beneficial effect on rats with menopausal syndrome caused by chronic stress stimulation and high-sugar and high-fat diet. GSNF promotes ovarian follicle development, inhibits follicle apoptosis, reduces ovarian damage, improves ovarian function, and improves menopausal syndrome by regulating the FSHR/PI3K/AKT signaling pathway. In addition, GSNF protects hippocampal neurons, promotes neurotransmitter secretion, and inhibits depression-like behavior. GSNF improves liver steatosis, reverses glucose and lipid metabolism disorders, and alleviates the adverse effects of negative emotions and bad eating habits on menopausal syndrome. In summary, the experimental data show that GSNF has the potential to treat or prevent menopausal syndrome.

Treatment of ulcerative colitis with Dendrobium huoshanense: network pharmacology and experimental validation

Abstract:

Dendrobium huoshanense (DH) is a traditional Chinese medicine with a long history of medicinal use. Combined with network pharmacology and in vivo experimental validation, the mechanism of action and mechanism of action of Dendrobium huoshanense in the treatment of ulcerative colitis (UC) were explored. Using network pharmacology methods, the mechanism of DH in the treatment of UC was preliminarily predicted through component screening, target prediction, PPI network interaction analysis, GO and KEGG enrichment analysis. And the 3% DSS-induced UC mouse model was used to verify its mechanism of action. Based on network pharmacology analysis, a total of 101 effective components were identified, of which 19 may be the key components for the effectiveness of DH in the treatment of UC. In addition, the study revealed 314 potential core therapeutic targets and the five most important targets: SRC, STAT3, AKT1, HSP90AA1, and PIK3CA. In the UC live mouse experiment, it was found that DH reduced the levels of IL-6 and TNF-α in the blood, while increasing the levels of IL-10 and TGF-β. This led to significant improvements in colon length and injury severity, and upregulated expression of SRC, STAT3, HSP90AA1, PIK3CA, p-AKT1, and PI3K/AKT signaling pathways in colon tissue. This study preliminarily predicted the effective ingredients and main targets of DH in the treatment of UC, and explored its possible mechanism of action through network pharmacology. These results provide an experimental basis for the clinical application of DH.

Conclusions:

In this study, a reduction in DSS-induced weight loss, diarrhea, colon tissue damage, blood in feces, and inflammatory response was observed when DH was administered. It may be related to the regulation of the expression of SRC, STAT3, AKT1, HSP90AA1, PIK3CA, and PI3K/AKT signaling pathways. In addition, it was found that DH may be involved in inhibiting the development of UC to CRC. The results will contribute to the application, development and exploitation of DH, and provide new ideas for the treatment of intestinal diseases. Thus, experimental support is provided for its traditional application.

Inhibitory effect of Dendrobium officinale polysaccharide on oxidative damage of glial cells in aged mice by regulating intestinal flora

Abstract:

Dendrobium officinale polysaccharide has multiple physiological effects. In this study, D-galactose-induced aged mice were used as experimental animal models to study the inhibitory effect of Dendrobium officinale polysaccharide (DOP) on oxidative damage of glial cells by alleviating oxidative stress and regulating intestinal microbiota. The results showed that DOP could significantly reduce the activation of glial cells in aged mice, increase the activity of antioxidant enzymes, and reduce the content of MDA. In addition, DOP reshaped the disordered intestinal flora, reduced the abundance ratio of Firmicutes to Bacteroidetes, and increased the abundance of the beneficial bacteria Lactobacillus. DOP may reverse the disorder of intestinal flora and alleviate the oxidative damage of glial cells, thereby exerting a potential neuroprotective effect by regulating intestinal flora.

Conclusions:

The study showed that D-galactose-induced intestinal flora destruction and glial cell overactivation in aged mice can lead to the massive expression of IDO1, Aβ, and Tau proteins. In contrast, DOP treatment can improve the prognosis of glial cell oxidative damage in aged mice by changing the composition of intestinal flora. The activation of glial cells is negatively correlated with certain intestinal flora, and their increase contributes to the body's antioxidant and anti-aging functions. However, this study has not fully elucidated the mechanism by which DOP treatment-mediated changes in intestinal flora regulate oxidative stress and aging-related signaling pathways. Therefore, it is necessary to further study the role of intestinal flora and its metabolites in the antioxidant and anti-aging functions of DOP from the perspective of molecular biology, which will help to fully reveal the mechanisms of DOP anti-aging and gut-brain axis.